Combined parental obesity negatively impacts preimplantation mouse embryo development, kinetics, morphology and metabolism
نویسندگان
چکیده
منابع مشابه
preimplantation mouse embryo
The role of membrane sterols in the compaction and subsequent development of the preimplantation mouse embryo was studied by incubating embryos in 7-ketocholesterol and other oxygenated sterols. These sterols have been shown to inhibit sterol synthesis and deplete membranes of cholesterol in a variety of other cell types. Compaction and subsequent blastocyst formation were normal when embryos w...
متن کاملThe Effect of Human Follicular Fluid (FF) on Preimplantation Mouse Embryo Development in Vitro
SUMMARY Since at oocyte retrieval for in vitro fertilization there is some FF become incorporate to egg, we decided to investigate the effect of this fluid on preimplantation embryo development in vitro. FF was obtained at the time of oocyte retrieval for IVF, centrifuged and then either inactivated by heat and stored at 4°c or without inactivation stored at-20°c until used. Late 2-cell emb...
متن کاملMembrane sterols and the development of the preimplantation mouse embryo.
The role of membrane sterols in the compaction and subsequent development of the preimplantation mouse embryo was studied by incubating embryos in 7-ketocholesterol and other oxygenated sterols. These sterols have been shown to inhibit sterol synthesis and deplete membranes of cholesterol in a variety of ther cell types. Compaction and subsequent blastocyst formation were normal when embryos we...
متن کاملFunctional Analysis of Lysosomes During Mouse Preimplantation Embryo Development
Lysosomes are acidic and highly dynamic organelles that are essential for macromolecule degradation and many other cellular functions. However, little is known about lysosomal function during early embryogenesis. Here, we found that the number of lysosomes increased after fertilization. Lysosomes were abundant during mouse preimplantation development until the morula stage, but their numbers de...
متن کاملFAM deubiquitylating enzyme is essential for preimplantation mouse embryo development
FAM is a developmentally regulated substrate-specific deubiquitylating enzyme. It binds the cell adhesion and signalling molecules beta-catenin and AF-6 in vitro, and stabilises both in mammalian cell culture. To determine if FAM is required at the earliest stages of mouse development we examined its expression and function in preimplantation mouse embryos. FAM is expressed at all stages of pre...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2015
ISSN: 0268-1161,1460-2350
DOI: 10.1093/humrep/dev142